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goat anti type iii collagen  (SouthernBiotech)


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    Structured Review

    SouthernBiotech goat anti type iii collagen
    Goat Anti Type Iii Collagen, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+type+i+collagen+antibody/Goat+Anti-Type+I+Collagen-UNLB/pm41855092-68-84-90
    Average 96 stars, based on 463 article reviews
    goat anti type iii collagen - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Nanoparticle-induced inflammation and fibrosis in ex vivo murine precision-cut liver slices and effects of nanoparticle exposure conditions.
    Article Snippet: Collagen 1 immunostaining After antigen retrieval, the sections were washed in 1 × PBS (phosphate buffered saline, pH 7) and circled using a DAKO pen (ImmEdge, Vector Laboratories inc.). .. A droplet of a 1:400 dilution of goat anti-type I collagen antibody (IgG, unlabeled, Southern Biotech) was added to the sections and incubated for 1 h at room temperature. ..

    Article Title: Inflammatory liver tissue formation using oxygen permeable membrane based culture platform.
    Article Snippet: 1389-1723/$ https://doi.or During chronic liver injury, inflammation leads to liver fibrosis, particularly due to the activation of hepatic stellate cells (HSCs).. The involvement of inflammatory cytokines in HSC activation and the interplay among different liver cells are elaborated.. To examine their interactions in vitro, many cultured liver tissue models are performed in organoid or spheroid culture with random 3D structure.

    Article Title: Nanoparticle-induced inflammation and fibrosis in ex vivo murine precision-cut liver slices and effects of nanoparticle exposure conditions
    Article Snippet: After antigen retrieval, the sections were washed in 1 × PBS (phosphate buffered saline, pH 7) and circled using a DAKO pen (ImmEdge, Vector Laboratories inc.). .. A droplet of a 1:400 dilution of goat anti-type I collagen antibody (IgG, unlabeled, Southern Biotech) was added to the sections and incubated for 1 h at room temperature. ..

    Article Title: Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect.
    Article Snippet: .. Immunohistochemistry was performed for Collagen type I and Collagen type II as described previously.25 Sections were treated with 4 mg/ml (Dako Pepsin S3002) for 30 min at room temperature for antigen retrieval prior to sequential incubation with a goat anti-type I collagen antibody (1:100, S1310-01; SouthernBiotech) or a mouse anti-type II collagen antibody (1:50, AF5710; Acris) at 4 C overnight followed by a biotinylated rabbit anti-goat secondary (1:1000, 305-065-003; Jackson ImmunoResearch Inc.) for collagen type I and a goat anti-mouse (1:1000; KPL 71-00-29) for collagen type II for 30 min at room temperature. ..

    Blocking Assay:

    Article Title: Inflammatory liver tissue formation using oxygen permeable membrane based culture platform.
    Article Snippet: 1389-1723/$ https://doi.or During chronic liver injury, inflammation leads to liver fibrosis, particularly due to the activation of hepatic stellate cells (HSCs).. The involvement of inflammatory cytokines in HSC activation and the interplay among different liver cells are elaborated.. To examine their interactions in vitro, many cultured liver tissue models are performed in organoid or spheroid culture with random 3D structure.

    Membrane:

    Article Title: Pulmonary siRNA Delivery with Sophisticated Amphiphilic Poly(Spermine Acrylamides) for the Treatment of Lung Fibrosis.
    Article Snippet: Per lane, 10 μg protein were separated on a 10% SDS polyacrylamide gel and transferred to a PVDF membrane (Roth, Karlsruhe, Germany). .. The membrane was blocked with 1% bovine serum albumin (BSA, Sigma–Aldrich) and treated with either rabbit anti-PAR-2 antibody (cat. no.: 35–2300, ThermoFisher Scientific, Dreieich, Germany) or goat anti-type I collagen antibody (cat. no.: 1310-01, SouthernBiotech, Birmingham, AL). .. Proteins were detected using the Amersham ECL Select Western Blotting Detection Reagent (GE Healthcare, Chicago, IL).

    Immunohistochemistry:

    Article Title: Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect.
    Article Snippet: .. Immunohistochemistry was performed for Collagen type I and Collagen type II as described previously.25 Sections were treated with 4 mg/ml (Dako Pepsin S3002) for 30 min at room temperature for antigen retrieval prior to sequential incubation with a goat anti-type I collagen antibody (1:100, S1310-01; SouthernBiotech) or a mouse anti-type II collagen antibody (1:50, AF5710; Acris) at 4 C overnight followed by a biotinylated rabbit anti-goat secondary (1:1000, 305-065-003; Jackson ImmunoResearch Inc.) for collagen type I and a goat anti-mouse (1:1000; KPL 71-00-29) for collagen type II for 30 min at room temperature. ..

    other:

    Article Title: Atorvastatin reduces renal interstitial fibrosis caused by unilateral ureteral obstruction through inhibiting the transcriptional activity of YAP.
    Article Snippet: Renal interstitial fibrosis is the primary pathological basis for the progression and development of various chronic kidney diseases, ultimately leading to renal failure.. Obstructive kidney disease caused by conditions such as kidney stones, is a common cause of renal fibrosis.. The Hippo pathway is a crucial signaling pathway that senses mechanical forces and is involved in the pathophysiology of fibrosis.



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    (a) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were collected for immunoblot. Left panel shows quantitative immunoblots, and right panel shows quantifications. N = 3 biological replicates. (b, e) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 6 biological replicates for (b), and N = 3 biological replicates for (e). (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblot to measure intracellular <t>collagen</t> <t>I</t> protein levels. Top panel shows representative immunoblots, and bottom panel shows quantifications. N = 3 biological replicates. (d) Cas9 scramble, sgCCPG1, and sgFAM134B II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I and LAMPI were stained with immunofluorescence of permeabilized cells. Top panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and bottom panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample, and colocalization was compared with a two-way ANOVA followed by a Tukey post-hoc. N = 5 biological replicates. (f) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 4 biological replicates. (g) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblotting. Left panel shows representative immunoblots, and right panel shows quantifications. N = 3 biological replicates. Samples were analyzed with a one-way ANOVA (a) or two-way ANOVA (b-g) followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.
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    cat 1310 08  (SouthernBiotech)
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    (a) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were collected for immunoblot. Left panel shows quantitative immunoblots, and right panel shows quantifications. N = 3 biological replicates. (b, e) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 6 biological replicates for (b), and N = 3 biological replicates for (e). (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblot to measure intracellular <t>collagen</t> <t>I</t> protein levels. Top panel shows representative immunoblots, and bottom panel shows quantifications. N = 3 biological replicates. (d) Cas9 scramble, sgCCPG1, and sgFAM134B II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I and LAMPI were stained with immunofluorescence of permeabilized cells. Top panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and bottom panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample, and colocalization was compared with a two-way ANOVA followed by a Tukey post-hoc. N = 5 biological replicates. (f) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 4 biological replicates. (g) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblotting. Left panel shows representative immunoblots, and right panel shows quantifications. N = 3 biological replicates. Samples were analyzed with a one-way ANOVA (a) or two-way ANOVA (b-g) followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.
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    Image Search Results


    (a) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were collected for immunoblot. Left panel shows quantitative immunoblots, and right panel shows quantifications. N = 3 biological replicates. (b, e) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 6 biological replicates for (b), and N = 3 biological replicates for (e). (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblot to measure intracellular collagen I protein levels. Top panel shows representative immunoblots, and bottom panel shows quantifications. N = 3 biological replicates. (d) Cas9 scramble, sgCCPG1, and sgFAM134B II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I and LAMPI were stained with immunofluorescence of permeabilized cells. Top panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and bottom panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample, and colocalization was compared with a two-way ANOVA followed by a Tukey post-hoc. N = 5 biological replicates. (f) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 4 biological replicates. (g) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblotting. Left panel shows representative immunoblots, and right panel shows quantifications. N = 3 biological replicates. Samples were analyzed with a one-way ANOVA (a) or two-way ANOVA (b-g) followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: FAM134B Controls Collagen I Dynamics in Hepatic Stellate Cell-Driven Fibrosis

    doi: 10.1152/ajpgi.00170.2025

    Figure Lengend Snippet: (a) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were collected for immunoblot. Left panel shows quantitative immunoblots, and right panel shows quantifications. N = 3 biological replicates. (b, e) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 6 biological replicates for (b), and N = 3 biological replicates for (e). (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblot to measure intracellular collagen I protein levels. Top panel shows representative immunoblots, and bottom panel shows quantifications. N = 3 biological replicates. (d) Cas9 scramble, sgCCPG1, and sgFAM134B II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I and LAMPI were stained with immunofluorescence of permeabilized cells. Top panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and bottom panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample, and colocalization was compared with a two-way ANOVA followed by a Tukey post-hoc. N = 5 biological replicates. (f) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours, and mRNA was collected for qRT-PCR. N = 4 biological replicates. (g) siControl, siCCPG1, and siFAM134B cells were treated with TGFβ (5 ng/mL) for 24 hours and collected for immunoblotting. Left panel shows representative immunoblots, and right panel shows quantifications. N = 3 biological replicates. Samples were analyzed with a one-way ANOVA (a) or two-way ANOVA (b-g) followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.

    Article Snippet: Antibodies used were as follows: Collagen I (Southern Biotech 1310–01), Collagen 5A1 (Cell Signaling Technologies 37304, RRID:AB_3675529), LAMP1 (Cell Signaling Technologies D2D11, RRID:AB_2687579), and Procollagen I (Millipore MAB1912, RRID:AB_94405).

    Techniques: Western Blot, Quantitative RT-PCR, Staining, Immunofluorescence, Confocal Microscopy, Standard Deviation

    (a) Cas9 scramble and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and collagen I deposition was stained with immunofluorescence of non-permeabilized cells. Left panel shows representative images (scale bar = 50μm), and right panel shows quantifications. Samples were imaged by confocal microscopy at 10x with at least 10 images per sample. Cas9 Control, sgCCPG1, and sgFAM134B I and II samples were compared with a two-way ANOVA followed by a Tukey post-hoc. N = 9 biological replicates. (b) LX-2 cells were transfected with scramble siRNA, siRNA against CCPG1, or siRNA against FAM134B (siControl, siCCPG1, and siFAM134B cells), and cells were treated with TGFβ (5 ng/mL) for 24 hours. Collagen I deposition was stained with immunofluorescence of non-permeabilized cells. Left panel shows representative images (scale bars = 30μm), and right panel shows quantifications. Samples were imaged by confocal microscopy at 20x with at least 10 images per sample. N = 7 biological replicates. (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and conditioned media samples were collected and concentrated for immunoblot. Left panel shows representative immunoblots, and right panel shows quantifications. N = 5 biological replicates. (d). LX-2 cells were treated with TGFβ (2ng/mL) for 16 hours, followed by CHX (50μM) ± CQ treatment (50μM). Conditioned media was harvested 4, 6, or 8 hours following CHX ± CQ addition and analyzed by immunoblotting. Coomassie staining for total protein serves as a control. Samples were analyzed with a two-way ANOVA followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: FAM134B Controls Collagen I Dynamics in Hepatic Stellate Cell-Driven Fibrosis

    doi: 10.1152/ajpgi.00170.2025

    Figure Lengend Snippet: (a) Cas9 scramble and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and collagen I deposition was stained with immunofluorescence of non-permeabilized cells. Left panel shows representative images (scale bar = 50μm), and right panel shows quantifications. Samples were imaged by confocal microscopy at 10x with at least 10 images per sample. Cas9 Control, sgCCPG1, and sgFAM134B I and II samples were compared with a two-way ANOVA followed by a Tukey post-hoc. N = 9 biological replicates. (b) LX-2 cells were transfected with scramble siRNA, siRNA against CCPG1, or siRNA against FAM134B (siControl, siCCPG1, and siFAM134B cells), and cells were treated with TGFβ (5 ng/mL) for 24 hours. Collagen I deposition was stained with immunofluorescence of non-permeabilized cells. Left panel shows representative images (scale bars = 30μm), and right panel shows quantifications. Samples were imaged by confocal microscopy at 20x with at least 10 images per sample. N = 7 biological replicates. (c) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and conditioned media samples were collected and concentrated for immunoblot. Left panel shows representative immunoblots, and right panel shows quantifications. N = 5 biological replicates. (d). LX-2 cells were treated with TGFβ (2ng/mL) for 16 hours, followed by CHX (50μM) ± CQ treatment (50μM). Conditioned media was harvested 4, 6, or 8 hours following CHX ± CQ addition and analyzed by immunoblotting. Coomassie staining for total protein serves as a control. Samples were analyzed with a two-way ANOVA followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.

    Article Snippet: Antibodies used were as follows: Collagen I (Southern Biotech 1310–01), Collagen 5A1 (Cell Signaling Technologies 37304, RRID:AB_3675529), LAMP1 (Cell Signaling Technologies D2D11, RRID:AB_2687579), and Procollagen I (Millipore MAB1912, RRID:AB_94405).

    Techniques: Staining, Immunofluorescence, Confocal Microscopy, Control, Transfection, Western Blot, Standard Deviation

    (a, b) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I (a) or procollagen I (b) were stained with immunofluorescence of permeabilized cells. Left panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and right panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample. Left panel shows representative images, and right panel shows quantifications. N = 7 biological replicates for (a), and N = 6 biological replicates for (b). Samples were analyzed with a two-way ANOVA followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05 and ** = p < 0.01.

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: FAM134B Controls Collagen I Dynamics in Hepatic Stellate Cell-Driven Fibrosis

    doi: 10.1152/ajpgi.00170.2025

    Figure Lengend Snippet: (a, b) Cas9 scramble, sgCCPG1, and sgFAM134B I and II cells were treated with TGFβ (5 ng/mL) for 24 hours, and intracellular collagen I (a) or procollagen I (b) were stained with immunofluorescence of permeabilized cells. Left panel shows representative images (scale bars = 10μm for left panels and 2.5μm for the insets), and right panel shows quantifications. Samples were imaged by confocal microscopy at 63x with at least 10 images per sample. Left panel shows representative images, and right panel shows quantifications. N = 7 biological replicates for (a), and N = 6 biological replicates for (b). Samples were analyzed with a two-way ANOVA followed by a Tukey post-hoc. Graph bars represent sample mean and error bars depict standard deviation; ns = not significant, * = p < 0.05 and ** = p < 0.01.

    Article Snippet: Antibodies used were as follows: Collagen I (Southern Biotech 1310–01), Collagen 5A1 (Cell Signaling Technologies 37304, RRID:AB_3675529), LAMP1 (Cell Signaling Technologies D2D11, RRID:AB_2687579), and Procollagen I (Millipore MAB1912, RRID:AB_94405).

    Techniques: Staining, Immunofluorescence, Confocal Microscopy, Standard Deviation

    (a) During HSC activation, COL1A1 mRNA is transcribed in the nucleus and translated into the ER for folding. Misfolded collagen I aggregates are trafficked to the lysosome for degradation through ER-phagy mediated by FAM134B, while folded collagen I is secreted from the cell into the extracellular space for deposition into collagen fibrils. (b) The loss of FAM134B prevents trafficking of misfolded collagen I aggregates to the lysosome. To restore ER homeostasis, we predict that misfolded collagen I is secreted from the cell, where it interferes with normal collagen fibril assembly and deposition. Created in BioRender. Maiers, J. (2025) https://BioRender.com/lu8v4nq .

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: FAM134B Controls Collagen I Dynamics in Hepatic Stellate Cell-Driven Fibrosis

    doi: 10.1152/ajpgi.00170.2025

    Figure Lengend Snippet: (a) During HSC activation, COL1A1 mRNA is transcribed in the nucleus and translated into the ER for folding. Misfolded collagen I aggregates are trafficked to the lysosome for degradation through ER-phagy mediated by FAM134B, while folded collagen I is secreted from the cell into the extracellular space for deposition into collagen fibrils. (b) The loss of FAM134B prevents trafficking of misfolded collagen I aggregates to the lysosome. To restore ER homeostasis, we predict that misfolded collagen I is secreted from the cell, where it interferes with normal collagen fibril assembly and deposition. Created in BioRender. Maiers, J. (2025) https://BioRender.com/lu8v4nq .

    Article Snippet: Antibodies used were as follows: Collagen I (Southern Biotech 1310–01), Collagen 5A1 (Cell Signaling Technologies 37304, RRID:AB_3675529), LAMP1 (Cell Signaling Technologies D2D11, RRID:AB_2687579), and Procollagen I (Millipore MAB1912, RRID:AB_94405).

    Techniques: Activation Assay